Born as the son of Valerio Marcano, a low-level and disgraced member of the Carillo Crime Family, Lucio lived with his father and brothers on a shed in River Row and was arrested along with his brothers Sal and Lou at a State Penitentiary at Cordoba for a series of robberies and crimes around New Bordeaux, while their youngest brother Tommy was on elementary school.
In 1934, Sal had his own father killed and blamed Giuseppe Carillo for the deed, convincing Lucio, Lou and their henchmen and followers to declare war on Carillo. That same year, while Carillo was dinning at a popular Italian restaurant on the French Ward, the Marcano brothers and their allies ambushed Carillo and shot the whole place down, causing the deaths of Carillo and 18 mobsters and guests.
After Carillo's death, the Marcanos took over New Bordeaux. After the takeover, Lucio started helping Lou organizing their Prostitution rackets around the city, building up some high-class and rich clientele in Frisco Fields. In the process, he ended up meeting and dating Olivia Grady, a young rich woman, and despite her parents despising Lucio due to him being a pimp and a pusher, he married her in 1949.
After that, Lucio got Olivia involved in the Marcanos' businesses, and would often take her with him on businesses involving fast cars, automatic guns and heavy booze. However, in 1962, Lucio was found dead with his throat slit, allegedly by one of his prostitutes. However, it is rumored that Olivia hired some men to kill him so she could take over his operations.
- Olivia Marcano (wife, deceased)
- Sal Marcano (brother, deceased)
- Lou Marcano (brother, deceased)
- Tommy Marcano (brother, deceased)
- Giorgi Marcano (nephew, deceased)
- Michael Grecco (nephew, deceased)
- Virginia Marcano (sister-in-law, deceased)
- Valerio Marcano (father, deceased)
- Sal Marcano stated that he had nightmares a month after Lucio died, suggesting that the two were close.
- Olivia Marcano described that when she first met Lucio, he was dashing and handsome, and only drove the fastest and best cars and wore the finest suits.
- Lucio is the only Marcano brother who wasn't killed by Lincoln Clay and also the only one not to make a physical appearance in the game.